Legume-rhizobia symbiosis enables symbiotic nitrogen fixation in root nodules, reducing fertilizer use and nitrogen pollution. Rhizobial infection occurs either intracellularly through root cells or intercellularly between them, and although the intercellular mode is presumed to be ancestral and mechanistically simpler, its genetic background remains largely unknown as suitable model systems have been lacking.  

By utilizing a newly identified model system, this project will discern host genetics involved in intra- vs. intercellular infection through single-cell RNA sequencing. Candidate genes specifically involved in intercellular infection will be further analyzed for nutritional regulation and conserved functions in the ancient symbiosis with arbuscular mycorrhizal fungi.

This work aims to improve our understanding of endophyte infection, plant organ formation and mechanisms regulating infection by commensal bacteria and pathogens. Furthermore, defining regulation by nutrient availability may contribute knowledge relevant to resource-efficient legume-based cropping systems, and investigation of conservation with an ancient symbiosis may provide insights that advance fundamental legume-rhizobia research.

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